This assay applies the power of next-generation sequencing (NGS) to gene expression profiling of typical clinical samples. Based on the TruSeq RNA Exome Library Prep Kit and Novogene’s in-house bioinformatics pipelines, it provides robust and comprehensive transcriptomic analyses of human RNA isolated from FFPE and other low-quality clinical samples.
FFPE RNA-Seq Service Overview
NovaSeq 6000, PE150
Data Quality Guarantee
We guarantee that ≥ 80% of bases have a sequencing quality score ≥ Q30, which exceeds Illumina’s official guarantee of ≥ 75%.
Recommended Sequencing Depth
40 M reads
FFPE RNA-Seq Bioinformatics Analyses Workflow
Compared to poly(A) RNA-Seq, this workflow is superior on multiple aspects of data quality metrics with higher percentage of clean data, lower error rate, higher Q30 and lower percentage of rRNA reads.
We have analyzed hundreds of FFPE samples with this RNA-Seq assay. Table 1 provides an example of the data quality metrics from those analyses.
Table 1. Examples of data quality metrics from FFPE samples analysed with this assay.
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